Caffeine

INTRODUCTION

Caffeine is the most widely used psychoactive substance and has been considered by some investigators as a drug of abuse. This article summarizes the available data on caffeine dependence, tolerance, reinforcement, and withdrawal. After sudden caffeine cessation, withdrawal symptoms develop in a small portion of the population, but these symptoms are moderate and transient.

Tolerance to caffeine-induced stimulation of locomotor activity has been shown in animals. In humans, tolerance to some subjective effects of caffeine may occur, but most of the time complete tolerance to many effects of caffeine on the central nervous system (CNS) does not occur. In animals, caffeine can act as a reinforcer but only in a more limited range of conditions than do classic drugs of dependence. In humans, the reinforcing stimulus functions of caffeine are limited to low or moderate doses, while high doses usually are avoided.

Classic drugs of abuse lead to specific increases in cerebral functional activity and dopamine release in the shell of the nucleus accumbens (the key neural structure for reward, motivation, and addiction). In contrast, caffeine at doses reflecting daily human consumption does not induce a release of dopamine in the shell of the nucleus accumbens but leads to a release of dopamine in the prefrontal cortex, which is consistent with its reinforcing properties.

Furthermore, caffeine increases glucose utilization in the shell of the nucleus accumbens only at high concentrations, which, in turn, nonspecifically stimulates most brain structures and thus likely reflects the side effects linked to high caffeine ingestion alone. Also, this dose is 5-10 times higher than the dose necessary to stimulate the caudate nucleus (extrapyramidal motor system) and the neural structures regulating the sleep-wake cycle, the 2 functions that are most sensitive to caffeine. Thus, although caffeine fulfills some of the criteria for drug dependence and shares with amphetamine and cocaine a certain specificity of action on the cerebral dopaminergic system, this methylxanthine does not act on the dopaminergic structures related to reward, motivation, and addiction.

Significant dietary sources of caffeine include coffee, tea, cola drinks, and chocolate. The most notable behavioral effects of caffeine occur after consumption of low to moderate doses (50-300 mg) and include increased alertness, energy, and ability to concentrate. Moderate caffeine consumption rarely leads to health risks. In contrast, higher doses of caffeine induce negative effects such as anxiety, restlessness, insomnia, and tachycardia. These effects are seen primarily in a small group of individuals who are caffeine sensitive. On the other hand, caffeine was considered in one study as a potential drug of abuse and more recently was described as a model drug of abuse. On the basis of a review of science and clinical data, the possibility of adding caffeine withdrawal, but not abuse and dependence, to diagnostic manuals is being considered in the United States.

Coffee and caffeine consumption

History

Caffeine was used mainly in the Arab world until the 15th century. It reached Europe during the 16th century, and its consumption spread rapidly. According to recent surveys, coffee consumption varies among countries. Consumption (more than 10 kg/person/year) is highest in Scandinavian countries (as well as in Austria and the Netherlands). In most Western European countries (as well as in Brazil and Costa Rica), coffee consumption ranges from 6-9 kg/person/year. The lowest consumption (less than 5 kg/person/year) occurs in the United States, Italy, Algeria, Nicaragua, and Paraguay. World coffee consumption is increasing.

The average consumption of coffee in 1990 was 1.41 cups per day in Japan, 1.73 cups per day in the United States, and 3.87 cups per day in Germany. In the United States, coffee consumption decreased in 1986 and has not changed significantly since then. In Japan, coffee consumption has been increasing constantly over the last 10 years, while in Germany consumption has been stable over the same period.

The results of a French survey indicate that 4 attitudes are linked positively to the quantity of coffee consumed. In decreasing order of importance they are (1) the need for a stimulant, (2) the preference for strong coffee, (3) the knowledge of coffee, and (4) the preference for the coffee roasting shop. The content of caffeine per cup of coffee varies and is dependent on the size of the serving, the mode of coffee preparation (eg, boiled, filter, percolated, espresso, instant), and the type of coffee used (eg, Arabica, Robusta). The size of a cup of coffee can range from 50-190 mL, and the standard caffeine content in a cup of coffee can be as low as 19 mg/cup for instant coffee and as high as 177 mg/cup in boiled coffee. The content of caffeine in a cup of coffee ranges from 0.7-1.1 mg/mL for boiled or filter coffee, 0.6-3.3 mg/mL for espresso, and 0.2-0.6 mg/mL (but can be as high as 1.0 mg/mL) for instant coffee.

Coffee types

The 2 major coffee types are Arabica and Robusta.

  • Arabica: Most of the coffee consumed is Arabica. It represents 70-100% of the total coffee consumed in Finland and Sweden. The content of caffeine expressed as percent of dry weight ranges from 0.9-1.2% in green Arabica beans and averages 1.3% in roasted Arabica beans.

  • Robusta: Robusta is consumed primarily in France, Italy, Portugal, and the United Kingdom, where it accounts for 42-70% of the total coffee consumption. The average content of caffeine is about twice as much in Robusta as in Arabica coffee. The caffeine content is 1.6-2.4% and 2.0% of the dry weight for green and roasted beans, respectively.

In a standard 150 mL cup, the content of caffeine ranges from 71-120 mg per cup for Arabica coffee and from 131-220 mg per cup for Robusta.

Caffeine consumption

Caffeine is present in a number of dietary sources including tea, coffee, cocoa beverages, candy bars, and soft drinks. The caffeine content of these food items varies, ranging from 71-220 mg/150 mL for coffee, 32-42 mg/150 mL for tea, 32-70 mg/330 mL for cola, and 4 mg/150 mL for cocoa. Average caffeine consumption from all sources is approximately 76 mg/person/day but reaches 210-238 mg/person/day in the United States and Canada and more than 400 mg/person/day in Sweden and Finland, where 80-100% of the caffeine intake is from coffee alone. In the United Kingdom, the consumption of caffeine is similar to that in Sweden and Finland, but 72% is from tea.

The daily intake of caffeine from all sources in the United States is estimated at 3 mg/kg/person, with two thirds of it coming from coffee consumed by subjects older than 10 years. If only caffeine consumers are evaluated, the daily caffeine consumption is 2.4-4.0 mg/kg (170-300 mg) in individuals weighing 60-70 kg. In children, soft drinks represent 55%, chocolate foods and beverages represent 35-40%, and tea represents 6-10% of the total caffeine intake.

PHARMACOLOGY OF CAFFEINE

Caffeine, or 1,3,7-trimethylxanthine, is related structurally to uric acid. It is metabolized by demethylation and oxidation. The major human pathway results in paraxanthine (1,7-dimethylxanthine), leading to the principal urinary metabolites, l-methylxanthine, 1-methyluric acid, and an acetylated uracil derivative. Minor degradation pathways involve the formation and metabolism of theophylline and theobromine. No evidence exists to suggest that methylxanthines are converted to uric acid or that their ingestion can exacerbate gout.

The rate of elimination of methylxanthines varies by individual due to both genetic and environmental factors, and 4-fold differences are not uncommon. In most cases, metabolism obeys first-order elimination kinetics within the therapeutic range. At higher concentrations, however, zero-order kinetics occur with the saturation of metabolic enzymes. This prolongs the decline of caffeine concentrations.

The metabolism of methylxanthines also is influenced by the presence of other agents or specific diseases. For example, cigarette smoking and oral contraceptives produce a small but appreciable increase in methylxanthine clearance. The half-life of theophylline can be prolonged significantly in patients with hepatic cirrhosis, congestive heart failure, or acute pulmonary congestion; values of more than 60 hours have been reported.

Caffeine has a half-life in plasma of 3-7 hours; this increases by about 2-fold in women during the later stages of pregnancy or with long-term use of oral contraceptive steroids. In premature infants, the rate of elimination of methylxanthines is quite slow.

CELLULAR BASIS FOR THE ACTION OF CAFFEINE

Three basic cellular actions of methylxanthines are probably responsible for their diverse effects. In order of increasing importance, they are (1) translocations of intracellular calcium, (2) increasing accumulation of cyclic nucleotides, and (3) adenosine receptor blockade.

The ability of methylxanthines to inhibit cyclic nucleotide phosphodiesterases often is cited to explain their therapeutic effects; however, strong evidence for this theory is lacking. Plasma caffeine concentrations that raise blood pressure are below the threshold for phosphodiesterase inhibition. Thus, phosphodiesterase inhibition is probably not important to the therapeutic effects of methylxanthines.

At high concentrations (0.5-1 mmol), caffeine interferes with the uptake and storage of calcium by the sarcoplasmic reticulum in striated muscles. This action can account for observations that such concentrations of caffeine increase the strength and duration of contractions in both skeletal and cardiac muscles. Similar actions can enhance secretion in certain tissues. However, their having an important role at therapeutic concentrations is unlikely. In vitro, methylxanthines (approximately 0.2 mmol or more) generally cause relaxation of vascular smooth muscles in the presence of various stimulators of contraction (eg, norepinephrine, angiotensin). While relaxation probably results from a reduction of the cytosolic calcium concentration, the extent to which methylxanthines can alter calcium binding and transport, either directly or indirectly, by altering cyclic nucleotide metabolism is unclear.

Thus, adenosine receptor blockade appears to be the predominant mode of action. Methylxanthines act as competitive antagonists at adenosine receptors at concentrations well within the therapeutic range. The effects of exogenous adenosine are frequently opposite to those of the methylxanthines, and the removal of ambient adenosine in some experimental settings (by the addition of adenosine deaminase) can reproduce the actions of the methylxanthines. Plasma concentrations of caffeine that raise blood pressure are within the range for antagonism of adenosine receptors.

Several other caffeine actions that have received relatively little attention to date might prove to be important for certain methylxanthine effects. These include their potentiation of inhibitors of prostaglandin synthesis and the possibility that methylxanthines reduce the uptake and/or metabolism of catecholamines in non-neuronal tissues.

 EFFECTS OF CAFFEINE ON THE CENTRAL NERVOUS SYSTEM

In animals, most of the pharmacological effects of adenosine in the brain can be suppressed by relatively low concentrations of circulating caffeine (less than 100 µmol, which is the equivalent of 1-3 cups of coffee). Adenosine decreases the neuronal firing rate and inhibits both synaptic transmission and the release of most neurotransmitters. Caffeine also increases the turnover of many neurotransmitters, including monoamines and acetylcholine.

The A1 and A2a adenosine receptors are the subtypes primarily involved in the caffeine effect, while A2b and A3 receptors play only a minor role. The A1 receptors are linked negatively to adenyl cyclase, while the A2a receptors are linked positively to this enzyme. Adenosine A1 receptors are distributed widely throughout the brain, with high levels in the hippocampus, cerebral and cerebellar cortex, and thalamus. Conversely, A2a receptors are located almost exclusively in the striatum, nucleus accumbens, and olfactory tubercle. In the latter regions, A2a receptors are coexpressed with enkephalin and dopamine D2 receptors in striatal neurons. Direct evidence exists for a central functional interaction between adenosine A2a and dopamine D2 receptors. Indeed, administration of adenosine A2a receptor agonists decreases the affinity of dopamine binding to D2 receptors in striatal membranes.

Interaction between adenosine A2a receptors and dopamine D2 receptors in the striatum might underlie some of the behavioral effects of methylxanthines. By antagonizing the negative modulatory effects of adenosine receptors on dopamine receptors, caffeine leads to inhibition and blockade of adenosine A2 receptors, causing potentiation of dopaminergic neurotransmission. The latter interaction might explain the adenosine receptor antagonists–induced increase in behaviors related to dopamine (eg, caffeine-induced rotational behavior).

CLINICAL TRIALS ON CAFFEINE: CENTRAL NERVOUS SYSTEM AROUSAL

In a Dutch study, event-related potentials were recorded from 11 subjects after administering caffeine (250 mg) or placebo. Subjects were instructed to attend selectively to stimuli with a specified color (red or blue) in order to react to the occurrence of a target within the attended category. Reaction times revealed faster responses in subjects who had been administered caffeine, whereas no differences in strategy were observed between the 2 groups. This study suggested that caffeine results in a higher overall arousal level, more profound processing of both attended and unattended information, and acceleration of motor processes.

In a study conducted by Bertini et al, preterm infants with a gestational age of less than 32 weeks and birth weight of less than 1500 g were randomized to receive either caffeine or aminophylline treatment for apnea of prematurity. This study concluded that caffeine does not significantly affect brain hemodynamics, while aminophylline induces a significant transient increase in oxygenated hemoglobin (HbO2) and cerebral blood volume (CBV).

Quinlan et al from the United Kingdom randomized subjects after overnight caffeine abstention. In the first study (n=17), the caffeine level was manipulated by preparing tea and coffee at different strengths (equivalent of 1-2 cups). Caffeine levels were 37.5 mg and 75 mg in tea and 75 mg and 150 mg in coffee, and the controls were given water or no drinks. In the second study (n=15), the caffeine level alone was manipulated (water or decaffeinated tea plus 0 mg, 25 mg, 50 mg, 100 mg, and 200 mg of caffeine). Beverage volume and temperature (55 degrees Celsius) were constant. Systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate, skin temperature, skin conductance, and mood were monitored over 3-hour study sessions.

In study 1, tea and coffee produced mild autonomic stimulation and mood elevation. Effects were not related to source of caffeine (tea versus coffee) or caffeine dose, despite a 4-fold variation in the latter. Increasing beverage strength was associated with greater increases in DBP and significant arousal. In study 2, caffeinated beverages increased SBP, DBP, and skin conductance, and lowered heart rate and skin temperature were noted in those who had water. Significant dose-response relationships to caffeine were seen only for SBP, heart rate, and skin temperature. Caffeine had significant effects on arousal but no consistent dose-response effects. The authors concluded that caffeinated beverages acutely stimulate the autonomic nervous system and increase alertness. In addition, caffeine can exert dose-dependent effects on a number of acute autonomic responses.

Leyner and Horn gave 200 mg caffeine or placebo to young truck drivers in a double-blind fashion. Caffeine significantly reduced sleep incidents for the first 30 minutes and reduced subjective sleepiness for an hour. This caffeine dose (via coffee) effectively reduced early morning driver sleepiness for about 30 minutes following sleep deprivation and for approximately 2 hours after sleep restriction.

Ligouri and Grass compared the effects of caffeine on subjective arousal in introverts and extraverts. Seventeen introverts and 19 extraverts drank coffee that contained caffeine doses of 0 mg/kg, 2 mg/kg, or 4 mg/kg during morning and evening sessions in a randomized, double-blind, cross-over design. At 30-minute intervals (for 180 min postcaffeine dose), participants completed the Profile of Mood States, a battery of visual analog scales, and the Digit Symbol Substitution Test (DSST). Caffeine effects on mood and task performance did not significantly affect extraversion, except for nonsignificant trends for caffeine to increase happiness and vigor (effect greater in extraverts than in introverts).

In a study by Herz, the effect of 5 mg/kg of caffeine or placebo on learning and retrieval sessions was studied, and mood was evaluated by several self-report measures. Sixteen words were studied during the learning session, and memory was evaluated by the number of words correctly recalled at the retrieval session 2 days later. Results revealed that caffeine reliably increased arousal, but it did not affect any emotion characteristics related to pleasure. Subjects who received caffeine at learning and retrieval were in equivalent mood states at both sessions. Moreover, caffeine did not produce any effects on memory; thus, neither hypothesis concerning the influence of arousal on memory was supported by these studies.

CAFFEINE AS A TREATMENT OF CONDITIONS RELATED TO THE CENTRAL NERVOUS SYSTEM

Migraine headaches

A retrospective study examined the benefits of the nonprescription combination of acetaminophen, aspirin, and caffeine (AAC), eg, Excedrin Migraine from Bristol-Myers Squibb Company, for the treatment of menstruation-associated migraine compared with migraine not associated with menses. Data were derived from 3 double-blind, randomized, placebo-controlled, single-dose trials enrolling subjects who met the International Headache Society's diagnostic criteria for migraine with or without aura. Subjects with incapacitating disability (ie, attacks requiring bed rest more than 50% of the time) or those who usually experience vomiting 20% or more of the time were excluded.

Retrospective analysis of the 1220 subjects included in the efficacy-evaluated data set indicated that 185 women were treated for menstruation-associated migraine, 781 women were treated for migraine not associated with menses, and one woman provided no information regarding menstrual status. At baseline and at 0.5, 1, 2, 3, 4, and 6 hours after treatment, subjects assessed the intensity of headache pain, functional disability, nausea, photophobia, and phonophobia. Pain intensity, nausea, photophobia, and phonophobia were rated on a 4-point scale (ie, 0-3, where 0=none and 3=severe) and functional disability was rated on a 5-point scale (ie, 0-4, where 0=none and 4=incapacitating).

For both menstruation-associated migraine and migraine not associated with menses, the proportion of subjects with pain intensity reduced to mild or none (ie, responders) was significantly greater with AAC than with placebo at all postdose time points from 0.5-6 hours (P< or = 0.05); treatment effect did not differ significantly between women with menstruation-associated migraine and women with migraine not associated with menses at any postdose time point.

Migraine characteristics, such as photophobia, phonophobia, and functional disability, were significantly improved in AAC-treated subjects at all time points from 1-6 hours (P< or = 0.01) in both groups, menstruating women and nonmenstruating women. Significant relief from nausea was experienced both by women with menstruation-associated migraine and by women with migraine not associated with menses, but relief appeared earlier in the nonmenstruating subjects given AAC (2 h postdose, P< or = 0.01) compared to menstruating subjects (6 h postdose, P< or = 0.05). Beginning at 3 hours after treatment, significantly fewer subjects treated with AAC required rescue medication (P< or = 0.05) for menstruation-associated migraine (AAC 6%, placebo 15%) and for migraine not associated with menses (AAC 7%, placebo 14%).

The most commonly used rescue medications in both the menstruating and nonmenstruating groups were nonsteroidal anti-inflammatory drugs, prescription combination analgesics/narcotics, and prescription migraine preparations. AAC was well tolerated both in women with menstruation-associated migraine and in women with migraine not associated with menses. In general, adverse experiences were similar in both the groups.

The proportion of subjects who had one or more adverse experiences was significantly higher among those receiving AAC than among those receiving placebo (menstruation-associated migraine: AAC 26.4%, placebo 12.6%, P= 0.025; migraine not associated with menstruation: AAC 18.6%, placebo 11.4%, P= 0.005). Adverse experiences were similar in type and severity to those previously associated with a single dose of acetaminophen, aspirin, or caffeine. Thus, the nonprescription combination of AAC was shown to be highly effective in treating the pain, disability, and associated symptoms of both menstruation-associated migraine and migraine not associated with menses.

CAFFEINE: THE QUESTION OF DEPENDENCE

Drug dependence is defined as a pattern of behavior focused on the repetitive and compulsive seeking and taking of a psychoactive drug.

The World Health Organization (WHO) and the American Psychiatric Association (APA) proposed a new set of criteria for dependence. The diagnosis of dependence requires the fulfillment of 3 (nonspecified) of the 6 WHO or 7 APA criteria. The 7 criteria of dependence as proposed by the APA in the Diagnostic and Statistical Manual of Mental Disorders, 4th edition (DSM-IV), are as follows:

  • Tolerance (not specified for severity)

  • Substance-specific withdrawal syndrome (psychic or physiological, not specified for severity)

  • Substance often taken in larger amounts or over a longer period than intended

  • Persistent desire or unsuccessful efforts to cut down or control use

  • A great deal of time spent in activities necessary to obtain, use, or recover from the effects of the substance

  • Important social, occupational, or recreational activities given up or reduced because of substance use

  • Continued intake despite knowledge of a persistent or recurrent physical or psychological problem that is likely to have been caused or exacerbated by the substance

The 6 criteria proposed by the WHO differ only slightly from those of the APA, with a different sequence, slightly different formulations, and the combination of criteria 5 and 6. Possible substances of abuse can be classified according to the number of criteria met and the severity of symptoms and the frequency of occurrence.

Potential model of drug of abuse

Of 166 caffeine users interviewed, 14% and 3% met the criteria for moderate and severe caffeine dependence, respectively. Telephone screenings performed on 99 subjects in the United States found that 16 individuals fulfilled 4 of the 7 criteria cited above and were thus considered dependent on caffeine. Dependence was not related to daily caffeine intake, which ranged from 129-2548 mg/day. The median daily caffeine intake for the caffeine-dependent individuals was 360 mg (40% had a daily intake of 300 mg or less).

However, despite the absence of current psychiatric disorders at the time of the study in most of the individuals (14 out of 16), 11 of the 16 persons diagnosed with caffeine dependence had a history of psychiatric disorders, including substance abuse disorders (10 subjects) and mood disorders (7 subjects). The prevalence of these disorders was higher in caffeine-dependent individuals than in the general population (50%). The tendencies of associations between caffeine, alcohol, and nicotine consumption, as well as between mood disorders and nicotine dependence, have been reported previously.

The 4 main factors to consider with regard to the question of dependence are withdrawal, tolerance, reinforcement, and dependence.

Withdrawal

Caffeine withdrawal in animals

Several reports show caffeine withdrawal signs in rats, cats, and monkeys. These signs include decreases in locomotor activity, operant behavior, and the reinforcement threshold for electrical brain stimulation. Other studies show changes in the time spent in various phases of slow-wave sleep and avoidance of a preferred flavor when the latter was paired with caffeine abstinence. The severity of caffeine withdrawal is dose dependent. A decrease in locomotor activity does not appear when caffeine doses lower than 67 mg/kg/day are substituted for water. The length of the decrease in locomotor activity also is dependent on the dose of caffeine and the duration of the treatment before water substitution. The latency of the onset of caffeine withdrawal effects occurs within 24 hours and peaks at 24-48 hours. The caffeine withdrawal–induced behavioral changes last a few days, except for the sleep-related signs that have been shown to last for as long as 30 days after the initiation of caffeine withdrawal.

Characterization of withdrawal symptoms in humans

Caffeine withdrawal results in typical symptoms. The most often reported symptoms are headaches; fatigue; weakness; drowsiness; impaired concentration; work difficulty; depression; anxiety; irritability; increased muscle tension; and, occasionally, tremor, nausea, and vomiting. Withdrawal symptoms generally begin 12-24 hours after sudden cessation of caffeine consumption and reach a peak after 20-48 hours. In some individuals, however, these symptoms can appear within only 3-6 hours and can last for one week.

Withdrawal symptoms do not relate to the quantity of caffeine ingested daily. For example, Strain et al showed that withdrawal symptoms occur in individuals consuming 129-2548 mg/day of caffeine. In the last decade, 2 studies suggested that caffeine withdrawal symptoms (but not caffeine abuse or dependence) should be added to the list of diagnoses recognized by the American Health System (ie, DSM-IV and International Classification of Diseases, 10th edition [ICD-10]).

Caffeine consumption, fasting, and headaches before and after surgical procedures are strongly positively correlated. For every increase in the usual daily consumption of 100 mg of caffeine (about a cup of coffee), the risk of headache immediately before and after surgery is increased by 12% and 16%, respectively, and also correlates with the duration of fasting. The risk of headaches is reduced in individuals who drink caffeine or receive caffeine tablets on the day of the surgery. Therefore, permitting patients who use caffeine and are undergoing minor surgical procedures to ingest preoperative caffeine may be advisable.

Weber reported that in a similar population, 40-48% of the patients already suffered regular headaches (at least weekly) at the time of surgery. Moreover, a relationship exists between caffeine withdrawal, development of headaches, and changes in cerebral blood flow. Cerebral blood flow velocities increase during withdrawal headaches, significantly decrease within 30 minutes of caffeine intake in all subjects, and return to baseline values after 2 hours. This recent study confirms several previous studies that suggested that increased blood volume might be involved in caffeine-withdrawal headaches. Caffeine withdrawal symptoms were even reported in newborns whose mothers were heavy coffee drinkers during pregnancy. The infants displayed irritability, increased emotional activity, and vomiting. Symptoms were present at birth but spontaneously disappeared after a few days.

Relief of abstinence symptoms by caffeine

Caffeine withdrawal symptoms disappear shortly after ingestion of caffeine. This effect is linked strongly to the psychological satisfaction related to the ingestion of caffeine; this is especially true for the first cup of the day. The potential reversal of caffeine withdrawal-induced headache and other symptoms by absorption of caffeine alone has been known for more than 50 years (multiple studies). The occurrence of headaches following substitution of decaffeinated coffee predicts subsequent caffeine self-administration. Caffeine content influences coffee consumption, and the beneficial effects of caffeine consumption on mood or alertness seem to encourage the consumption of coffee or caffeine-containing beverages.

Heavy consumers of coffee show a preference for coffee containing caffeine, while those who typically drink decaffeinated coffee generally choose either decaffeinated or caffeine-containing coffee. When subjects are categorized as caffeine choosers and nonchoosers, caffeine choosers tend to report both positive subjective effects of caffeine (stimulant and positive effects on mood and vigilance) as well as negative subjective effects of placebo (headache and fatigue), while caffeine nonchoosers tend to report negative effects of caffeine (anxiety and dysphoria).

Tolerance

Tolerance to a drug refers to an acquired change in responsiveness after repeated exposure to the drug. Tolerance can be considered in 2 ways. First, tolerance might indicate that the dose necessary to achieve the desired euphoric or reinforcing effects increases with time, thus encouraging increased consumption of the drug. Second, tolerance to the aversive effects of high doses of the drug may occur, also leading to increased consumption of the drug over time.

Tolerance to many behavioral effects of caffeine occurs in mice, cats, and squirrel monkeys treated regularly with methylxanthine. Tolerance to caffeine-induced locomotor stimulation, cerebral electrical activity, reinforcement thresholds for electrical brain stimulation, schedule-controlled response maintained by presentation of food, and electric shock and thresholds for caffeine- or N-methyl D-aspartate (NMDA)-induced seizures has been described.

Development of tolerance to caffeine in animals is rapid, usually insurmountable, and shows cross-tolerance with the other methylxanthines but not with other psychomotor stimulants such as amphetamines and methylphenidate. On the first 2 days after caffeine discontinuation, depression of locomotor activity is noted with a return to baseline values on the third day (consistent with a withdrawal syndrome). Although the exact mechanism underlying the development of tolerance to caffeine remains unclear, tolerance to behavioral effects of caffeine in animals does not seem to involve adaptive changes in adenosine receptors but may result from compensatory changes in the dopaminergic system as a result of chronic adenosine receptor blockade.

In humans, the tolerance to some physiological actions of caffeine can occur. This is the case for the effect of caffeine on blood pressure, heart rate, diuresis, plasma adrenaline and noradrenaline levels, and renin activity. Tolerance usually develops within a few days. Tolerance to some subjective effects of caffeine, such as tension-anxiety, jitteriness, nervousness, and the strength of drug effect, has been shown. Conversely, although tolerance to the enhancement of arithmetic skills by caffeine was shown recently, evidence of tolerance to caffeine-induced alertness and wakefulness is limited. These effects are paralleled by the lack of tolerance of cerebral energy metabolism to caffeine, since acute administration of 10 mg/kg caffeine induces the same metabolic increase whether the rats have been exposed to previous daily treatment with caffeine or saline for 15 days.

Thus, every single exposure to caffeine can produce cerebral stimulant effects. This is especially true in the areas that control locomotor activity (eg, caudate nucleus) and structures involved in the sleep-wake cycle (eg, locus ceruleus, raphe nuclei, reticular formation). In humans, sleep seems to be the physiological function most sensitive to the effects of caffeine. Generally, more than 200 mg of caffeine is required to affect sleep significantly. Caffeine has been shown to prolong sleep latency and shorten total sleep duration with preservation of the dream phases of sleep. Whether the difference in the sensitivity to the effects of coffee on sleep can be attributable to tolerance is not clearly established.

According to some studies, this difference could reflect the individual sensitivity to caffeine, possibly related to differences in the rate of caffeine metabolism. Indeed, poor sleepers are reported to metabolize caffeine at a lower rate. Four of the 10 subjects of the study had elimination half-lives exceeding 4.8 hours. The variability in response from one night to the next also should be taken into account. Nevertheless, some evidence exists of tolerance to caffeine-related sleep disturbance, since heavy coffee drinkers appear to be less sensitive to caffeine-induced sleep disturbances than light coffee drinkers. Likewise, tolerance to sleep latency and quality of caffeine has been shown to develop over 2 days of testing in one study and over 7 days in another. However, the tolerance is not complete and the sleep efficiency remains below 90% of the baseline value after 7 days of caffeine treatment.

Thus, tolerance to some of the effects linked to regular consumption of coffee seems to occur, especially in animals. In humans, the data are less conclusive. This may be the result of individual differences in the susceptibility and tolerance to caffeine-induced effects. Moreover, mechanisms of tolerance may be overwhelmed by the nonlinear accumulation of caffeine and its primary metabolites in the human body when caffeine metabolism is saturated under multiple dosing conditions.

Discrimination and reinforcement

Discrimination of caffeine

Human subjects are able to discriminate caffeine against placebo, both when dosed in capsules and in coffee. The effects of doses of 300 mg or higher are detected more easily and primarily are recognized by negative effects, such as jitteriness, anxiety, or nervousness, whereas lower doses are detected by their lack of effect or by caffeine withdrawal symptoms. However, several studies have failed to demonstrate behavioral effects of caffeine at doses less than 200-300 mg (ie, amounts corresponding to ingestion of 2-3 cups of coffee). The effects of doses in the range of 100 mg, which closely approaches the caffeine content of a normal serving, were difficult to detect in one study, and were detected by 30-60% of the individuals in 2 other studies. However, in most of these studies, subjects were not withdrawn from their habitual daily intake of caffeine for a prolonged period of time; thus, tolerance may have played a role.

In fact, doses of caffeine below 100 mg do not induce withdrawal or negative effects, have rarely been shown to alter self-reports of mood or performance, and usually are preferred by moderate coffee drinkers. One study showed discrimination of caffeine at doses as low as 10 mg for one subject, 18 mg for 3 subjects, and 56 mg for 3 other subjects. This study, which involved the authors themselves, was replicated with subjects less informed of the potential effects of the drugs. Data in the same range as in the previous study were obtained with caffeine discrimination in one subject at 18 mg of caffeine, in one subject at 32 mg, in 2 subjects at 56 mg, and in 4 subjects at 100 mg caffeine.

The authors suggested that in specific individuals, caffeine could be considered to affect mood at doses lower than those previously reported. Indeed, some groups were able to show enhanced auditory vigilance and reaction time at caffeine doses of 75 mg, 64 mg, or even 32 mg. It seems that the effects of caffeine are utilized consciously or unconsciously by various individuals in the management of the mood state relevant to the context of drink choice. The discrimination of low doses of caffeine is not related to the taste of caffeine because at the dose of 100 mg, subjects are not able to reliably differentiate decaffeinated coffee plus lactose from decaffeinated coffee plus 100 mg of caffeine. Conversely, at higher doses, caffeine could be detected in coffee because higher concentrations are related directly to coffee bitterness.

Reinforcing effects of caffeine

Reinforcing efficacy of a drug refers to the relative efficacy in establishing or maintaining a behavior on which the delivery of the drug is dependent. In animals, intravenous self-administration of caffeine has been studied after the implantation of venous catheters that allows self-administration of the drug by lever pushing and assessment of behavioral reinforcement. In 4 studies, caffeine was shown to be self-injected in all animals, while 3 studies showed that only a limited subset (25-33%) of the animals self-administered caffeine. A sporadic pattern of caffeine self-administration, characterized by periods with high rates of self-injection alternating with periods of rather low intake, was found in nonhuman primates.

Thus, although caffeine seems to be able to act as a reinforcer in some conditions, a marked difference exists between caffeine and classic drugs of abuse that maintain self-administration across species and conditions (eg, amphetamines, cocaine). Recently, caffeine was shown to be able to reinstate extinguished cocaine-taking behavior in rats. This effect was more marked when caffeine was given for one day as opposed to 4 days following the last cocaine self-administration session. Thus, extended withdrawal increases the priming effects of caffeine. Note, however, that these animal studies use intravenous self-administration, while human caffeine consumption is always by oral route, and the former mode of administration is well known to be by far more addictive than the latter. For these reasons, caffeine does not appear to be a robust reinforcer in animals.

In humans, the widely recognized behavioral stimulant and mildly reinforcing properties of caffeine are probably responsible for the maintenance of caffeine self-administration, primarily in the form of caffeinated beverages, such as coffee, tea, and cola. In some studies, the choice of caffeine has been shown to be controlled more potently by avoiding withdrawal than by its positive effects, while other data support the hypothesis that the true performance-enhancing effects of caffeine are responsible for its self-administration. Most data showed that caffeine reinforcement occurs in 100% of heavy caffeine consumers (1020-1530 mg/d) who also have a history of alcohol or drug abuse. For moderate caffeine users (128-595 mg/d), caffeine reinforcement occurs in 45-100% of subjects.

Caffeine reinforcement varies with dose. Doses of caffeine in tea and coffee are high enough to act as reinforcers since these doses can induce withdrawal symptoms. A dose of 25-50 mg caffeine per cup of coffee acts as a reinforcer, while increasing doses beyond 50 or 100 mg tends to decrease the choice of caffeine or the frequency of caffeine self-administration; high doses of caffeine (400-600 mg in a single dose) are avoided. Caffeine reinforcement also relates to withdrawal symptoms after the cessation of coffee. Subjects who consistently suffer from caffeine withdrawal headache have a 2.6 times higher chance of selecting caffeinated coffee (containing 100 mg caffeine). Caffeine consumption is used to avoid withdrawal more than it is used for its positive effects.

Bickel reviewed 16 studies dealing with the behavioral economics paradigm for the study of drug abuse. Increasing consumption of a fixed-price item when a similar item becomes more expensive indicates a substitutive function and is seen clearly with opiates, cocaine, and phencyclidine but not with caffeine. These data confirm that caffeine is less reinforcing than amphetamine and related psychomotor stimulants.

Reinforcing effects of caffeine-containing drinks unrelated to caffeine

The conditions under which caffeine functions as a reinforcer are still not clearly understood. However, the possible reinforcing effects of coffee unrelated to caffeine include its aroma, taste, and social environment in which it is consumed. Subjects with a habitual coffee consumption of 4-10 cups per day (mean intake 6 cups per day) were switched, without a withdrawal period, to the consumption of 600 mg of caffeine, either in tablets containing 50 mg of caffeine each or decaffeinated instant coffee for 3 days. The desire for coffee in the next 3 days largely increased in the group given caffeine tablets but remained unchanged in the group given decaffeinated instant coffee, although the latter group experienced marked caffeine withdrawal symptoms.

The question of whether the taste of coffee and caffeine may influence its intake is still a matter of debate. If water-containing caffeine is given regularly to rats between 29 and 40 days of postnatal life, these rats, as adults, will drink more caffeinated water than tap water. Likewise, the previous administration of an adenosine agonist increases caffeine intake.

Thus, caffeine intake could, at least partly, be related to its pharmacological properties, though the influence of taste cannot be eliminated. In fact, coffee and caffeine have 2 components, appetitive and aversive. The absorption of low quantities of caffeine could favor the appetitive effect of caffeine, whereas higher quantities could exacerbate its aversive effects. In humans, the gustatory response to caffeine is not influenced by previous exposure to a series of methylxanthines, adenosine, or caffeine deprivation. However, the taste of coffee is an important aspect of caffeine consumption; subjects prefer caffeine in coffee to caffeine in capsules.

Also, another possibility is that caffeine is a constituent of coffee and tea, which are liked by users for reasons independent of their caffeine content. Thirst may be one factor but is probably not a major factor in the consumption of tea or coffee (though it could contribute to the consumption of soft drinks). An affinity for sensory properties of tea and coffee also can be related to the nutritional benefit derived from the milk, cream, and/or sugar added to the beverage.

The last possibility is the influence of situational conditions on mood that can play an important role in reinforcing preferences for specific foods and beverages. Indeed, coffee and tea often are consumed in social contexts and during breaks from work. Therefore, the influence of caffeine on the consumption of tea, coffee, or soft drinks may be relatively subtle and depends both on the cumulated dose over the day and the mood state in which it is consumed. For example, if an individual is already quite stimulated, ingestion of a caffeine-containing beverage may lead to unpleasant effects. Recent findings showed that, in some individuals, the choice to drink coffee is influenced by the interaction between the mood state before drinking coffee and the anticipated effects based on the content of caffeine in the drink.

CONCLUSIONS

Caffeine is contained in some of the most widely consumed foods and beverages, both in the United States and internationally. For this reason, it has been extensively investigated in both animal models and human studies. Although caffeine shares some characteristics with other chemicals of abuse with regard to both psychological and physiological dependence, important differences exist, especially pertaining to the action of caffeine in CNS neurotransmitter systems.

Clearly, further studies are required to better define both the short- and long-term roles of caffeine in the neurological and cardiovascular systems. The combined results of these future studies will be of keen interest to all those who enjoy that warm cup (or multiple cups) of coffee to start the morning.

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